bacterial tag-encoded flx 454-pyrosequencing Search Results


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Main characteristics of the studies included in this review. NI, No information
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Main characteristics of the studies included in this review. NI, No information
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Main characteristics of the studies included in this review. NI, No information

Journal: Annals of Clinical Microbiology and Antimicrobials

Article Title: Direct metagenomics investigation of non-surgical hard-to-heal wounds: a review

doi: 10.1186/s12941-024-00698-z

Figure Lengend Snippet: Main characteristics of the studies included in this review. NI, No information

Article Snippet: (Wolcott et al., 2009) , USA , Prospective , Ulcer debris , 40 , 16s metagenomics , centrifugation at 14,000 rpm for 30 s and resuspended in 500 μl RLT buffer (Qiagen), 5 mm steel bead (Qiagen), 500 μl sterile 0.1 mm glass beads, TissueLyser (Qiagen), and run at 30 Hz for 5 min. , QIAamp DNA Mini Kit (Qiagen) , No , , tag-encoded FLX and Titanium amplicon pyrosequencing (Roche) , Roche/454 GS FLX Titanium platform , NET and C# analysis pipeline with BLASTn , NCBI Bacterial database..

Techniques: Sample Prep, DNA Extraction, Sequencing, Centrifugation, Sterility, Amplification, Selection, DNA Library Preparation, DNA Purification, Software, Incubation, Polymerase Chain Reaction